Application

Western Blotting Analysis: A representative lot detected TMEM16B in 293-EBNA cell lysates overexpressing TMEM16B and in mouse retina and brain homogenates (Stohr, H., et al. (2009). The Journal of Neuro. 29(21):6809-6818).
Immunocytochemistry Analysis: A representative lot detected TMEM16B in mouse retina cells (Stohr, H., et al. (2009). The Journal of Neuro. 29(21):6809-6818).

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Anti-TMEM16B Antibody, clone 7H10 is a highly specific mouse monoclonal antibody, that targets TMEM16B & has been tested in IHC, western blotting & ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The protein named Anoctamin-2 or alternatively Transmembrane protein 16B and encoded by the human gene ANO2/C12orf3/TMEM16B is a calcium activated chloride channel that plays a role in smell or olfactory signal transduction and photoreception. Odorant molecules bind to odor-sensing receptors leading to an increase in calcium entry that then activates TMEM16B calcium current which amplifies the depolarization of the Odor-sensing receptors. TEMEM16B also mediates light perception amplification in the retina where TMEM16B is a component of the presynaptic protein complex recruited to specialized plasma membrane domains of photoreceptors. TEMEM16B is expressed in sensory neurons particularly in the retina and olfactory neurons; it localizes to the plasma membrane where it is a multi-pass membrane bound protein. TEMEM16B has multiple isoforms and recent research suggest that the native channel may be composed of two or more splicing isoforms acting as subunits that together shape the channel properties.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of human TMEM16B.

Epitope: N-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Immunohistochemistry in mouse retina tissue.

Imunnohistochemistry Analysis: A 1:50 dilution of this antibody detected TMEM16B in mouse retina tissue.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

114 kDa calculated